Laboratories of microscopy

Potential utilization of the laboratories


There are two kinds of microscopy we focus on in our laboratories. The first is the optical microscopy and the other is atomic microscopy. Both microscope types are adjusted for material science.
AFM laboratory, that is the atomic forces microscopy, focuses on three-dimensional surface projection, the main topic are thin films, polymer-composites,… Optical microscopy is on our department mainly used as a mean of quality estimation for soldered or conductive adhesive-made joints, material degradation or quality control of printed circuit boards.

During the lessons, students are presented crosssections of SMD components, they can see the different print quality of commercial printers, and so on.

Laboratories instrumentation

Laboratories instrumentation is periodically renewed, therefore, students can see not just devices, that are already surpassed bu curent state of the art - though these devices still have their utilization on our department - but also instruments, that are used lately.

AFM microscope Veeco di Innova


AFM microscopy is based on sample surface atom forces mapping. AFM microscope rasterizes the sample using a sharp sensing probe, positioned at an end of an loose arm (cantilever). The mutual force interaction between tip and sample surface causes the cantilever to deflect and bend. The proximity of the tip to the surface causes the attractive (Van der Waals) and repulsive forces (arising from the Pauli principle) to bend the arm. This bending is sensed by a sensitive laser sensor and the computer generates the surface topography of the sample. It is essential, that the surface forces arise at any sample independently on its conductivity.
There are altogether three modes in which the AFM microscope can operate: the contact mode, the non-contact mode and so called tapping mode.

Optical Microscope Olympus SZX7


This microscope is a direct optical microscope with magnification of up to 120x. It is specifically adjusted to prevent the observed sample from being damaged by electrostatic discharge.
This optical microscope is in our laboratory supplemented by Olympus E-330 digital camera. This mutual connection, together with QuickPHOTO Industrial software allows easy image processing and measuring of required data.
There are two types of light used for the sample illumination. It is either LED light, that can be fixed directly on the microscope lens, or external source of cold light (Oympus KL 1500 LCD), which has been specially designed for applications in stereo microscopy and macroscopy. By combining both lights, quality data acquisition is achieved.

Camera microscope Neophot 32


Camera microscope Neophot 32, made by VEB Carl Zeiss Jena is an inverted material science microscope. The magnification capability is up to 2000x and the carrying capacity for the sample is 5 kg. The source of light is a halogen lamp or a xenon lamp, which are both integrated into the device. In order to view the sample, either a projection screen or micro-photographic device can be used.
Since 2003, the microscope was enriched with Nikon CoolPix 5700, that was attached using specially made-up adapter. The images can then be easily observed and further processed on a computer. During lessons, a TV screen is connected to the camera, so that samples can be observed by wider audience and in real time.

Optical microscope JENAVERT


Optical stereomicroscope JENAVERT is again a VEB Carl Zeiss Jena product. The reflected light is used for sample illumination. The magnification is 1000x. JENAVERT is used particularly to assess the material microstructure.

Lower magnification microscopes


Subjects taught in laboratories

You, as a student can work on above mentioned equipment during the followind lessons: A0M13KTM (Konstrukce a technologie mikropočítačů), X13KTM (Konstrukce a technologie mikropočítačů), A0B13NNT (Nanotechnologie), AE0B13NNT (Nanotechnology) a A1B14SEM (Elektrotechnický seminář), or you can work in these laboratories during your student project, bachelor or diploma work. Microscope laboratories on our department are also widely used by our doctoral students.

Examples of specific work

Selected results from the AFM microscope:


Selected results from the optical microscope:


GPS coordinates of the laboratories

Laboratories T2:B3:141b and T2:B3:141c, where you can find microscopes of our departmentve, are located on the first floor of the building of the Faculty of Electrical Engineering in Dejvicke in the corridor opposite the cantine.


GPS coordinates T2:B3-141b: 50°06'10.65"N, 14°23'35.40"E
GPS coordinates T2:B3-141c: 50°06'10.75"N, 14°23'35.50"E

Responsible for information: Lenka Hájková, David Bušek

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